Sharjah Chemicals is leading Supplier, Manufacture and Exporter of Sudan IV in Sharjah UAE, Al Qusais, Deira, Saif Zone, Fujairah,Dubai, Abudhabi, Ajman, Diera, UAE Middle East, Al Quoz, Jebel Ali, Umm Al Quawain, Ras Al Khaimah, Masafi, Khor Fakkan, Al Hayl, Al Ain, Ras Al Khor Industrial Area, Al khubaisi, Um Ramool Industrial Area, Al Qusais Industrial Area, Jebel Ali.
Product Information:
SUDAN IV : RXSOL-69-8583-600
Sudan IV (C24H20N4O) is a lysochrome (fat-soluble dye) diazo dye used for the staining of lipids, triglycerides and lipoproteins on frozen paraffin sections. It has the appearance of reddish brown crystals with melting point 199 °C and maximum absorption at 520(357) nm.
Product Application:
- Sudan IV has been used industrially to color oils, waxes, shoe polish, greases, oily-resin lacquers, varnishes, cellulose acetate and acrylic resins, and in wood stains. As a biological stain, it is used primarily to stain lipids and fatty substances present in cells and tissues. The color imparted is a function of the solubility of the dye in the substance affected and does not involve chemical bonding. A saturated solution in isopropanol is prepared for use as a fat stain in animal tissue.
- Sudan IV is one of the dyes used for Sudan staining. Similar dyes include Oil Red O, Sudan III, and Sudan Black B. Staining is an important biochemical technique, offering the ability to visually qualify the presence of the fatty compound of interest without isolating it. For staining purposes, Sudan IV can be made up in propylene glycol. Alternatively, authors have reported using the dye saturated in isopropyl alcohol, 95% ethanol, or 0.05% by weight in acetone:ethanol:water (50:35:15). The idea is to use a moderately apolar solvent to solubilize the dye allowing it to partition into the highly apolar fat without the solvent solubilizing the fat to be stained.
Leave a Reply